Isolation of sperm in insects

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We describe here a simple and efficient protocol for genomic DNA isolation from adult males of insects: e. To minimize contamination of external DNA source, the sperm vesicles were isolated from male individuals from which high molecular weight genomic DNA was extracted. According to this protocol, the genomic DNA samples obtained were high quality intact , and abundant enough for genotyping analyses and molecular cloning. The protocol reported here enables us to process a huge number of individuals at a time with escaping from cross-contamination, and thus it is quite useful for conducting genetic studies at least in some species of insects. The large yield of high molecular weight DNA from single individual may be advantageous for non PCR-based experiments.
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Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Takemon and A. Yamamoto and Masashi Nakashima and K.
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